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FAQ You can easily check the FAQ for answers to any questions you may have.

  • FIV suspected cat came to our hospital, but rapid Ab test was negative. After that, the cat died. How can we explain this to pet owner?

    Q: FIV suspected cat was tested and rapid kit result was negative .But the cat died, how should I explain the case  to the owner.

    A.Rapid Ab test may show negative results in very initial or terminal phase. It is known that in case of FIV, the antibody begin to develop after 60 days of exposure in most cats, and development of detectable antibodies may be considerably delayed in some cats. In terminal cases, compromised immune system result in lack of antibody development which may show negative results regardless of severe clinical signs. The latter fits in this case. We recommend you to repeat test minimum of 60 days after the last potential exposure.

  • Why cats should be tested FIV Ab before vaccination?

    FIV vaccination induce humoral immunity for antibodies to develop, and normal antibody test does not distinguish vaccination and natural infection.(Our Bionote FIV Ab kit can differentiate the natural infection from vaccination because it only detect the Ab produced by natural infection) In adult case, vaccine history or other DIVA method should be check for misdiagnosis. There are many cases of misdiagnosis of infection in uninfected cats which may lead to inappropriate changes in lifestyle or even euthanasia. 

  • Why FIV test does not have an Ag test?

    Cats infected with FIV have low levels of viral antigen in their blood, which has prevented the use of in-clinic screening assays based on antigen detection as used in diagnosis of feline leukemia virus (FeLV). However, cats with FIV generally produce high levels of antiviral antibodies. Since FIV produces a persistent, lifelong infection, detection of antibodies in peripheral blood of unvaccinated cats is considered sufficient for routine diagnostic screening. 

  • Is it possible that Anigen FIV Ab test to differentiate field infection and after vaccination?

     Anigen Rapid FIV Ab Test Kit differentiate the antibodies induced by natural infection and vaccination both. 

  • There is no migration

    Especially, FeLV Ag test kit requires 2 drops of assay buffer. If the dropping volume is not enough, the migration will not appear within the testing time (at 10 minutes) 

  • Does FeLV vaccination affects FeLV Ag rapid test result?

    Since FeLV tests are based on detection of antigen or DNA and not antibodies, vaccination against FeLV does not generally interfere with testing. However, blood collected immediately following vaccination may contain detectable FeLV antigens or DNA circulating from the vaccine itself. Therefore, it is adequate that samples are collected prior to FeLV vaccine administration. The duration of vaccine interference with testing is unknown. 

  • How long does it take to get FeLV positive result after infection?

    These tests are usually positive by 30 days after exposure; however, development of antigenemia is variable and may take considerably longer period. Retesting after 30 days is recommended for FeLV suspected case with negative result. 

  • Maternal infection during pregnancy

    Q: A Female cat came to our hospital and diagnosed with FeLV. The cat delivered kittens few  days ago, and the FeLV Ag test results of the kittens were negative. Are the negative test result reliable?

    A: Kittens may be tested at any time because passively acquired maternal antibody does not interfere with testing for viral antigen. However, kittens infected as a result of maternal transmission may not test positive for weeks to months after birth. Thus, the test results for kittens are not reliable in this case. Generally, cats should have negative test results for both FeLV and FIV prior to being introduced to group housing. So retest is preferable after few months.

  • FeLV Ag rapid test result conflicts IFA test result.

    Q: I conducted FeLV Ag rapid test and the result was positive. I requested IFA test for further confirmation, and the test result was negative. How should I interpret these test results? 

    A:Discordant test results occur when results of antigen screening tests and confirmatory IFA tests do not agree, making determination of the true FeLV status difficult. The most common scenario that occurs is like described above. Antigen test showing positive and the IFA test showing negative. In general, antigen tests are considered to be slightly more sensitive (fewer false negatives), and the IFA to be slightly more specific (fewer false positives). With either test, however, the reliability (predictive value) of the test is dependent on the prevalence of infection within different populations of cats. Cats with discordant test results are best considered potential sources of infection for other cats until their status is clarified. The true infection status may eventually be resolved by repeating both tests after 60 days and annually thereafter until the test results agree. Since cats with regressive infection are only transiently antigenemic, some cats are expected to revert to negative status on soluble antigen tests without ever having a positive IFA test

  • Why do the cats should be tested for FeLV before vaccination?

    There are two reasons for this. 

    1. Vaccine interference: There had been some reports about FeLV vaccines causing false positive which is called vaccine interference effects on cats right after vaccination. 

    2. Useless for already infected cats: Before vaccination, cats should be tested for the FeLV infection in advance because FeLV vaccine to the cats that are already infected with FeLV is ineffective.