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FAQ You can easily check the FAQ for answers to any questions you may have.

  • How about vaccine effect of FPV Ag kit detection?

    Unlike canine parvovirus vaccine of dog, feline panleukopenia virus vaccine can affect rapid FPV kit, According to article, there are different virus shedding competence for different commercial vaccine, and detect ability was also different for each commercial kit. It seems like vaccine interference period is about 1 week of post vaccination. So care should be done for result interpretation for FPV diagnosis for cat which vaccinated recently. 

  • A kitten came to our hospital for neurologic signs (ataxia). History and other situation indicated

    Q.A kitten came to our hospital for neurologic signs (ataxia). History and other situation indicated FPV infection. But the rapid kit result turned out to be negative. Is this test result reliable?

    A.That`s because of the short virus shedding period of feline panleukopenia virus. If the cat showed neurologic signs, which indicates late period of infection, it is likely that the virus shedding is over. So FPV Ag kit does not reveal the virus infection in this case. There are many causes of neurologic signs in kittens, so consider other causes that should be checked. FPV vaccine history should also be checked.

  • Is canine parvovirus infection possible in cat? And is it also possible that Anigen rapid kit can de

    Q. Is canine parvovirus infection possible in cat? And is it also possible that Anigen rapid kit can discriminate between the two virus?  

    A.Canine parvovirus can infect cat. Clinical signs of CPV infection in cat are almost same with FPV infection but severity is different. Generally, CPV infection in cat is mild and chance of survival is higher than FPV infection. Since the two viruses has high genetic homology, Anigen FPV Ag kit can also detect CPV infection in cat, but Practitioner should aware that CPV infection in cat has very limited virus shedding period (2~5 days), Care should be taken for test result interpretation. 

     

  • How about virus shedding period of feline panleukopenia virus for rapid kit use?

    Virus shedding period of feline panleukopenia infection is relatively short. Unlike parvovirus infection in canine which has 1 month of shedding period, feline panleukopenia virus shedding occurs approximately 2~10 days of exposure. Understanding this difference is important for diagnosis of FPV 

  • Causes of False Positive for FPV Ag test kit?

    1.In terms of utero infection, the affected litter can be clinically healthy probably due to their innate resistance or the acquisition of maternally derived antibodies(MDAs). Still, these kittens can harbor the virus for up to 2 months after birth. (Csiza et al., 1971a) 

    2.Not all cats infected with FPV develop clinical signs and the severity depends on age, immune status, and concurrent infections (Foley et al., 1999) 

    3.Cats can test positive for up to 3 weeks after modified live vaccination (Neuerer et al., 2008) 

    4.The greatest fecal virus shedding corresponds with the peak of clinical disease, but keep in mind that the virus can be shed for up to six weeks after recovery7 and that subclinical animals can also shed virus. (Greene et al., 1998) 

  • When can Anigen CHW Ag test kit show different result with other diagnostic method?

    1. In case of early infection stage: Generally, the immunochromatographic assay kit can detect microfilaria from 8 months of age because normally, a microfilaria grow up to become an adult worm until 7 months after infection -> false negative can be seen. 

    2. In case of infection induced by only male or female Dirofilaria: In this case, the microfilaria cannot be made in the blood and therefore cannot be observed under the microscope. -> They may show negative result with modified knott test or PCR which detects only microfilaria, but Anigen CHW rapid kit will show is as positive. 

    3. In case of Ivermectin treatment (the Ivermectin can kill the microfilaria): Microfilaria cannot be shown in the blood -> (Knott test: negative, Rapid test: positive) 

    4. Because of immune response of body, microfilaria can be disappeared -> (Knott test: negative, Rapid test: positive) 5. When the customer read the result after 20 minutes, the result can change from negative to false positive. So, we would like to recommend you to Interpret test results at 5 ~ 10 minutes. Do not decide after 20 minutes. 

  • How can Anigen Rapid CHW Ag Test Kit detect all male, female and microfilaria?

    Anigen CHW Ag kit using Heartworm-specific 14 kDa Ag is a common microfilaria antigen and adult worm including male and female. Even though our kit use the anti-14 kDa Ag monoclonal antibody, it is very hard to detect in microfilaria-infected L3, L4 and L5 stage serum because of low antigen concentration. 

  • If the test checked metabolite antigen, can the test result be confused with canine dipetalonema?

    Anigen Rapid CHW Ag Test Kit detects 14 KDa of Dirofilaria immitis antigen which is canine heartworm-specific antigen. So it would have no cross reaction with canine dipetalonema. 

  • There is no migration

    Anigen Rapid CHW Ag Test Kit use 2 drops of canine serum, plasma or whole blood. If the sample is inadequate as being clotted, the migration will not start within testing time (10 minutes) 

  • What antigen is detected by Anigen Rapid CHW Ag Test Kit (metabolite of HW or anything)?

    Anigen Rapid CHW Ag Test Kit detects 14 kDa protein secreted from D. immitis. The 14 kDa Ag is common antigen secreted from circulating microfilaria and adult female and male worm. The monoclonal antibody against 14 KDa Ag of D. immitis is used as detector and captures materials of HW Ag kit.