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Support

FAQ You can easily check the FAQ for answers to any questions you may have.

  • I used your Anigen Rapid CHW Ag 2.0 test kit on a patient positive for microfilaria, having had two
    Immiticide you`ve treated destroys only adult worm and does not affect microfilaria. So under the microscopic finding, microfilaria will be shown. Microfilaricidal therapy should be considered in this case, because the mechanism of action of microfilaricide is different from that of adulticide. The Anigen Heartworm Ag Test Kit can detect the 14kDa protein secreted from adult worm or microfilaria. But Microfilaria only secrets small amount of 14 kDa protein in the blood. Therefore, our kit will show negative result if the specimen has only microfilaria
  • Sample (Conjunctiva, Saliva) storage after collection and before testing

    Distemper virus (in conjunctiva, saliva) is unstable. For that reason, after collecting the specimen using cotton swab, the specimen should be immediately extracted and tested. If specimen could not be immediately used, it should be refrigerated at 2~8℃, for the storage not less than 48 hours. And if the specimen is freezeed at -20℃ or below, it will be stable for 1 year. 

  • If a dog has been treated with hyper immunoglobulin serum while suffering from canine distemper, wou

    Q. If a dog has been treated with hyper immunoglobulin serum while suffering from canine distemper, would CDV Ag rapid test result turn out to be negative?

    A.We have experience about hype-immune serum therapy at animal clinics. However, detailed experiments did not carried out. In our experience, CDV antigen in conjunctiva was detected at 1 day after hyper immune sera (SN: above 1024) administration (0.2ml/kg). But, the antigen had disappeared at 5-6 days after administration. In this case, we concluded that IgG did not neutralize the CDV in epithelial cells. That is, CDV can be detected from 1 to 6 days after hyper immune therapy due to the preexisted virus in epithelial cells. Anigen CDV Ag kit can help you to follow disease progress. If the virus disappeared from conjunctiva after therapy (hyper-immune sera, antibiotics etc), regarding to the test result of our kit, the dog may have had survived due to your optimal therapy. If our kit gave a negative result in case of CDV Ag presence (by PCR or other confirmation methods), we could consider it as false negative. However, hyper-immune sera therapy dose not directly influence CDV in conjunctiva. Conclusively, hyper-immune sera dose not influence CDV Ag test results. If the dog gave a negative, it means that the CDV antigen was disappeared in the body, especially in the conjunctiva.

  • Some customers tell me that test line appeared 10 minutes after the sample loading, and does that me

    Q: Some customers tell me that test line appeared 10 minutes after the sample loading, and does that mean the amount of antigen is low or disturbed by other agent? And they always continue asking how they can make the interpretation. If we tell the pet owner that the result is negative, how can we explain the faint test line which had appeared?

    A:First, we guess that the virus titer was very low, but the dog is suspected case of CDV positive because the test line appeared within 10 minutes. And if the clinical signs show the CDV infection, it would be considered as CDV positive. If there is no CDV clinical signs, dog should be retested after 3~4 days and if the retesting result is negative, the dog is CDV negative or has recovered from the infection.

  • I read the FAQ which indicated that making the swab wet by the assay diluents of CDV Ag kit is wrong
    Yes, Some chemicals in the assay diluents will make irritation to eyes. But the dog cannot say he is irritated on his eye. For that reason, the insert sheet is informing that the swab should be wet using the saline solution. We guess that most of pet clinic keep the saline solution usually
  • Why there’s no C line in CDV Ag tests?

    We recommend that test kit should be used according to instructions. Check the sample collection, storage, amount, or procedure of test. In our experience, there is no or faint control line in very low case (about 0.1%) especially, due to the high amount of samples. Generally, this phenomenon is non-specific due to the samples. In this case, retest with another testing method. 

  • What factors would affect the test result?

    Specimen collection, storage, amount, or procedure of the test could affect the result. Especially, the dropping speed and volume are very important. Please drop one by one with 2~5 seconds interval in every dropping. If a customer take too much sample on the swab, the mixed diluents become denser and denser. These dense diluents hinder the migration of fluid inside cassette. Therefore, we recommend the optimal sample collection. The best sample collection amount is slight smearing on the surface of cotton swab. 

  • Where is the correct site for conjunctiva swab?

    For CDV Ag specimen, before a veterinarian collects the conjunctiva swab, swab should be wetted by normal saline. And swab side to side (right and left eyes) in conjunctiva tissue is enough for specimzen sampling. 

  • A dog in other clinic, the CDV Ag was positive, the owner didn’t believe it. So they came to us, the

    Q: A dog in other clinic, the CDV Ag was positive, the owner didn’t believe it. So they came to us, the test was negative, after treatment it was tested again and showed positive result. The owner thought it’s our responsibility. Why it comes?

    A:We think that sampling had some problems. Sometimes, test results of the same dog show different band density on the same day depending on. In this case, each samples might had different virus titer depending on the sampling site.

  • Will vaccination influence result? If yes, how long after vaccination can we perform the test? If no

    Q.Will vaccination influence result? If yes, how long after vaccination can we perform the test? If no, is it because of different strain or other methodology? 

    A.Vaccine interference (Vaccine virus shedding) effect has been reported. Duration of post-vaccine interference is variable but may last as long as 3 weeks. However, it was confirmed by molecular based assay (Restriction fragment length polymorphism analysis) which is more sensitive than immunochromatographic assay. So Anigen Rapid CDV Test Kit does not likely to detect vaccine virus because virus titer is too low to be detected by rapid kit.