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FAQ You can easily check the FAQ for answers to any questions you may have.

  • Which Antigen does the Anigen FIV Ab test detects?

    Our FIV Ab test detects IgG antibodies against FIV. 

  • FIV & FeLV testing before adopting kitten

     Q: Kitten came to our hospital and owner wanted to test for FIV & FeLV before bringing them to their house.The result of  FIV Ab  was positive, FeLV Ag was negative. There was no other clinical signs. Should I consider this case as FIV positive? 

    A: Positive FIV antibody tests in kittens are unreliable. Antibodies from FIV-infected or FIV-vaccinated queens are passed to kittens in colostrum. These antibodies often persist past the age when many kittens are adopted into new homes and first tested for retroviral infection. Kittens are considered to be at low risk of infection, even when their mothers harbor the virus. Most kittens that test positive for FIV antibodies are not truly infected and will eventually revert to a seronegative status. It is recommended that kittens with positive FIV antibody tests be segregated from other cats and then undergo retesting every 1 to 2 months. Kittens that remain seropositive at 6 months are considered to be infected. Although postponing testing of kittens for FIV until they are over 6 months of age may be tempting, all kittens and cats should be tested before they are brought into new homes. The vast majority of kittens test negative and can be declared free of FIV infection.

  • FIV suspected cat came to our hospital, but rapid Ab test was negative. After that, the cat died. How can we explain this to pet owner?

    Q: FIV suspected cat was tested and rapid kit result was negative .But the cat died, how should I explain the case  to the owner.

    A.Rapid Ab test may show negative results in very initial or terminal phase. It is known that in case of FIV, the antibody begin to develop after 60 days of exposure in most cats, and development of detectable antibodies may be considerably delayed in some cats. In terminal cases, compromised immune system result in lack of antibody development which may show negative results regardless of severe clinical signs. The latter fits in this case. We recommend you to repeat test minimum of 60 days after the last potential exposure.

  • Why cats should be tested FIV Ab before vaccination?

    FIV vaccination induce humoral immunity for antibodies to develop, and normal antibody test does not distinguish vaccination and natural infection.(Our Bionote FIV Ab kit can differentiate the natural infection from vaccination because it only detect the Ab produced by natural infection) In adult case, vaccine history or other DIVA method should be check for misdiagnosis. There are many cases of misdiagnosis of infection in uninfected cats which may lead to inappropriate changes in lifestyle or even euthanasia. 

  • Why FIV test does not have an Ag test?

    Cats infected with FIV have low levels of viral antigen in their blood, which has prevented the use of in-clinic screening assays based on antigen detection as used in diagnosis of feline leukemia virus (FeLV). However, cats with FIV generally produce high levels of antiviral antibodies. Since FIV produces a persistent, lifelong infection, detection of antibodies in peripheral blood of unvaccinated cats is considered sufficient for routine diagnostic screening. 

  • Is it possible that Anigen FIV Ab test to differentiate field infection and after vaccination?

     Anigen Rapid FIV Ab Test Kit differentiate the antibodies induced by natural infection and vaccination both. 

  • There is no migration

    Especially, FeLV Ag test kit requires 2 drops of assay buffer. If the dropping volume is not enough, the migration will not appear within the testing time (at 10 minutes) 

  • Does FeLV vaccination affects FeLV Ag rapid test result?

    Since FeLV tests are based on detection of antigen or DNA and not antibodies, vaccination against FeLV does not generally interfere with testing. However, blood collected immediately following vaccination may contain detectable FeLV antigens or DNA circulating from the vaccine itself. Therefore, it is adequate that samples are collected prior to FeLV vaccine administration. The duration of vaccine interference with testing is unknown. 

  • How long does it take to get FeLV positive result after infection?

    These tests are usually positive by 30 days after exposure; however, development of antigenemia is variable and may take considerably longer period. Retesting after 30 days is recommended for FeLV suspected case with negative result. 

  • Maternal infection during pregnancy

    Q: A Female cat came to our hospital and diagnosed with FeLV. The cat delivered kittens few  days ago, and the FeLV Ag test results of the kittens were negative. Are the negative test result reliable?

    A: Kittens may be tested at any time because passively acquired maternal antibody does not interfere with testing for viral antigen. However, kittens infected as a result of maternal transmission may not test positive for weeks to months after birth. Thus, the test results for kittens are not reliable in this case. Generally, cats should have negative test results for both FeLV and FIV prior to being introduced to group housing. So retest is preferable after few months.