A paper on the analytical validation of Vcheck cCortisol has been published in the international journal, Medycyna Weterynaryjna (Science Citation Index Expanded). 

See the attached file for the full paper. 

(Paper: Medycyna Weterynaryjna 77(08):6562-2021) 

In dogs, serum cortisol concentration can be useful for the diagnosis of adrenal and pituitary disorders. Interpretation of serum cortisol concentration is crucial in the diagnosis and management of dogs with both hyperadrenocorticism and hypoadrenocorticism.

In this study conducted at University of Life Sciences in Lublin, Poland, non-fasting blood samples were obtained from 44 dogs.

Cortisol results obtained by the Vcheck analyzer were compared to those obtained by the IMMULITE 2000 immunoassay, which had previously been validated for the measurement of serum cortisol concentration in dogs.

As a result, Pearson’s Correlation analysis shows a very high consistency of the results obtained by the two analyzers (r=0.94, Fig. 1). The Bland-Altman test of agreement demonstrated that the Vcheck produced results close to those obtained by the reference method (Fig. 2).

According to this study, the results of the Vcheck method were comparable to those of the IMMULITE 2000 reference method, which makes it suitable for canine serum cortisol analysis for the diagnosis of adrenal disease. 

Vcheck cCortisol can be quantitatively measured using a fluorescent immunoassay analyzer, Vcheck, in a short time (20 minutes) and can be tested using a small amount of sample (serum 50 µl). It also has a wide measurement range of 1 to 30 µl/dl.