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QCan I detect microfilaria with Heartworm Antigen Test Kits?A
No. You can't detect microfilaria with our Test Kits. you can dectect abult worms.
You may dbeserve the micropliaria withe a microscope.
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QWhat antigen is detected by Bionote Rapid CHW Ag Test Kit (metabolite of HW or anything)?A
Bionote Rapid CHW Ag Test Kit detects 14 kDa protein secreted from D.immitis
The 14 kDa Ag is common antigen secreted from circulating microfilaria and adult female and male worm.
The monoclonal antibody against 14 kDa Ag of D.immitis is used as detector and captures materials of HW Ag kit.
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QHow much can I fill up the EDTA tube with the blood?A
The volume of blood you can fill in the EDTA tube is about 1 ml because of the volume of EDTA is about 20 μl.
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QI used your Bionote Rapid CHW Ag 2.0 test kit on a patient positive for microfilaria, having had two series of immiticide injections two days in a row per series. I rechecked the blood a month after the immiticide injection. Whereas the Ag test showedA
Immiticide you`ve treated destroys only adult worm and does not affect microfilaria. So under the microscopic finding, microfilaria will be shown.
Microfilaricidal therapy should be considered in this case, because the mechanism of action of microfilaricide is different from that of adulticide.
The Bionote Heartworm Ag Test Kit can detect the 14kDa protein secreted from adult worm or microfilaria. But Microfilaria only secrets small amount of 14 kDa protein in the blood.
Therefore, our kit will show negative result if the specimen has only microfilaria Or in case of low antigen concentration such a small number of adult worm,
the antigen can’t be detected with our test kits due to diagnostic limits.