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FAQ
  • QWill the FeLV Ag kit detect FeLV from vaccinations?
    A

    Since FeLV tests are based on detection of antigen or DNA and not antibodies, vaccination against FeLV does not generally interfere with testing. However, blood collected immediately following vaccination may contain detectable FeLV antigens or DNA circulating from the vaccine itself. Therefore, it is adequate that samples are collected prior to FeLV vaccine administration. The duration of vaccine interference with testing is unknown.

  • QHow long does it take to get FeLV positive result after infection?
    A

    These tests are usually positive by 30 days after exposure; however, development of antigenemia is variable and may take considerably longer period. 

    Retesting after 30 days is recommended for FeLV suspected case with negative result.

  • QWe have positive results with FeLV test for a cat, so we sent the sample to the laboratory for IFA test. But it was negative, how can we interpret the results?
    A

    Discordant test results occur when results of antigen screening tests and confirmatory IFA tests do not agree, making determination of the true FeLV status difficult. The most common scenario that occurs is like described above. Antigen test showing positive and the IFA test showing negative. In general, antigen tests are considered to be slightly more sensitive (fewer false negatives), and the IFA to be slightly more specific (fewer false positives). With either test, however, the reliability (predictive value) of the test is dependent on the prevalence of infection within different populations of cats. Cats with discordant test results are best considered potential sources of infection for other cats until their status is clarified. The true infection status may eventually be resolved by repeating both tests after 60 days and annually thereafter until the test results agree. Since cats with regressive infection are only transiently antigenemic, some cats are expected to revert to negative status on soluble antigen tests without ever having a positive IFA test

  • QWhy do the cats should be tested for FeLV before vaccination?
    A

    A: There are two reasons for this. 

     

    1. Vaccine interference: There had been some reports about FeLV vaccines causing false positive which is called vaccine interference effects on cats right after vaccination. 

     

    2. Useless for already infected cats: Before vaccination, cats should be tested for the FeLV infection in advance because FeLV vaccine to the cats that are already infected with FeLV is ineffective.

  • QThere is no migration
    A

    Especially, FeLV Ag test kit requires 2 drops of assay buffer. If the dropping volume is not enough, the migration will not appear within the testing time (at 10 minutes)